CORD BANK™

Collection, processing, and “long-term” cryopreservation of mesenchymal stem cells from cord tissue Wharton’s Jelly tissue (Wharton's Jelly is the tissue surrounding the two arteries and the one vein of the cord).

The procedure consists:

Collection:

Cord tissue samples are being processed immediately upon receive. The procedure protocol has been used in international research trials and has been certified after many tests. This procedure is carried out under absolute sterile conditions in the clinical laboratory.

Processing:

The cord tissue sample is being coded with a unique barcode for its traceability both during the processing and after the cryopreservation. Sample processing is being carried out immediately upon receive with validated procedures in a completely closed system (biogenea® cryoset). In ...

The cord tissue sample is being coded with a unique barcode for its traceability both during the processing and after the cryopreservation. Sample processing is being carried out immediately upon receive with validated procedures in a completely closed system (biogenea® cryoset). In particular, the mesenchymal stem cells are being isolated from cord tissue through enzymatic digestion and centrifugation. The whole procedure is being carried out in a laminar flow class A/B (Annex I - February 2008 EU guideline for the cGMP standards).

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Quality Control:

A cell count is being performed both prior to and after the procedure. Prior to the procedure a full mesenchymal stem cell test is being performed. The mesenchymal stem cell analysis is carried out using the automated flow cytometry analyzer. After the procedure completion, a small amount of ...

A cell count is being performed both prior to and after the procedure. Prior to the procedure a full mesenchymal stem cell test is being performed. The mesenchymal stem cell analysis is carried out using the automated flow cytometry analyzer. After the procedure completion, a small amount of the final product is being used for the final numerical counting of the progenitor cells, as well as their viability prior to the cryopreservation using flow cytometry SOPs protocol. A small amount of the sample is being cryopreserved and thawed especially for a viability test of the mesenchymal stem cells after the cryopreservation procedure. Using this procedure we can guarantee that the viability and the plasticity of the mesenchymal stem cells are intact during the cryopreservation. The viability test is being performed using the automated luminometric system, while the plasticity test is being performed using the Mesencult system. A small amount of the stem cell culture is controlled for the presence of aerobic and anaerobic germs using the automated blood culture system, while in parallel the new automated genetic analyzer is used for the detections of HBsAg, as well as for Hepatitis C, HIV Ι/ΙΙ , Toxoplasma , Syphilis and CMV.

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Long term Cryopreservation & Product Release:

Only after the completion of all the quality tests isolated mesenchymal stem cells are stored in temperatures reaching the -196C ( in these conditions all the metabolic procedures are stopped ) and document/certificate of cryopreservation is issued.

What can you do with it:

Due to the continuous advances in the future applications of the mesenchymal stem cells for therapeutic uses, the fact that these cellular populations are being used for many years for the treatment of immune diseases as well as a supporting treatment for some cancers, is being ignored. The ...

Due to the continuous advances in the future applications of the mesenchymal stem cells for therapeutic uses, the fact that these cellular populations are being used for many years for the treatment of immune diseases as well as a supporting treatment for some cancers, is being ignored. The progenitor mesenchymal cell from the Wharton’s Jelly is exclusively for autologous use. These cells can potentially be used for the treatment of many diseases such as the Parkinson's disease, the myocardial damages, the renal regeneration, and for the regenerations of many tissues and organs. The progenitor mesenchymal cells are known to have the ability to go to the damaged tissues and that's possible to be used in the future for the tissue regeneration. For the time being, the progenitor mesenchymal cells, due to their low level of immunological response can be used during the transplantation procedure for the avoidance of the graft versus host disease (GVHD).

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